SCON—a Short Conditional intrON for conditional knockout with one-step zygote injection
DESCRIPTION
Abstract
The generation of conditional alleles using CRISPR technology is still challenging.
Here, we introduce a Short Conditional intrON (SCON, 189 bp) that enables the rapid generation of conditional alleles via one-step zygote injection. In this study, a total of 13 SCON mouse lines were successfully generated by 2 different laboratories.
SCON has conditional intronic functions in various vertebrate species, and its target insertion is as simple as CRISPR/Cas9-mediated gene tagging.
