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SCON—a Short Conditional intrON for conditional knockout with one-step zygote injection



The generation of conditional alleles using CRISPR technology is still challenging.

Here, we introduce a Short Conditional intrON (SCON, 189 bp) that enables the rapid generation of conditional alleles via one-step zygote injection. In this study, a total of 13 SCON mouse lines were successfully generated by 2 different laboratories.

SCON has conditional intronic functions in various vertebrate species, and its target insertion is as simple as CRISPR/Cas9-mediated gene tagging.


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