How it works?
Senza categoria

Antithrombin III as predictive indicator of survival

Laura Bergantini, Miriana d'Alessandro and others.

Internal Medicine Journal


BAL samples were filtered through sterile gauze to remove mucus. Cell count was performed by cytocentrifuge smear (600 rpm for 5 min) using May-Grunewald Giemsa staining (DiaPath, Italy).

Cell viability was determined by Trypan blue exclusion. Flow cytometric analysis was performed using monoclonal antibodies (BD Multitest™ 6-color TBNK, San Jose, CA, USA), including CD3 FITC-labelled, CD16/CD56 PE-labelled, CD45 PerCP-Cy5.5-labelled, CD4 PE-Cy7-labelled, CD19 APC-labelled and CD8 APC-Cy7-labelled. About 1x106 cells were incubated with antibodies for 30 minutes, and then washed with 500 μl RPMI-1640 medium.


Diapath Lab Talks | Privacy Policy