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COVID-eVax, an electroporated DNA vaccine

Antonella Conforti, Emanuele Marra and others

Molecular Therapy


COVID-eVax is an electroporated vaccine that can finally be able to help us defeating this new virus that caused a lots of deaths.

The COVID-19 pandemic caused by SARS-CoV-2 has made the development of safe and effective vaccines a critical priority.

To date, four vaccines have been approved by European and American authorities for preventing COVID-19, but the development of additional vaccine platforms with improved supply and logistics profiles remains a pressing need.

Here we report the evaluation of a novel COVID-19 vaccine based on the electroporation of engineered, synthetic cDNA encoding a viral antigen.

We constructed a set of prototype DNA vaccines expressing various forms of the SARS-CoV-2 spike (S) protein and assessed their immunogenicity in animal models.

Among them, COVID-eVax—a DNA plasmid encoding a secreted monomeric form of SARS-CoV-2 S protein receptor-binding domain (RBD)—induced the most potent anti-SARS-CoV-2 neutralizing antibody responses (including against the current most common variants of concern) and a robust T cell response.

Upon challenge with SARS-CoV-2, immunized K18-hACE2 transgenic mice showed reduced weight loss, improved pulmonary function, and lower viral replication in the lungs and brain.

COVID-eVax conferred significant protection to ferrets upon SARS-CoV-2 challenge.

In summary, this study identifies COVID-eVax as an ideal COVID-19 vaccine candidate suitable for clinical development. Accordingly, a combined phase I-II trial has recently started.


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