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Laser-assisted bioprinting for bone regeneration

Nicolas Touya



Laser-assisted bioprinting can be a new way to improve the regenerative medicine, in particular the bone regeneration.

Grafts aside, current strategies employed to overcome bone loss still fail to reproduce native tissue physiology.

Among the emerging bioprinting strategies, laser-assisted bioprinting (LAB) offers very high resolution, allowing designing micrometric patterns in a contactless manner, providing a reproducible tool to test ink formulation.

To this date, no LAB associated ink succeeded to provide a reproducible ad integrum bone regeneration on a murine calvaria critical size defect model.

Using the BioRoot as an addition to a collagen-enriched ink, the present study describes the development of a tricalcium silicate-based ink.

This ink formulation was mechanically characterized by rheology to adjust it for LAB.

Printed aside stromal cells (SCAPs), this ink demonstrated a great compatibility, with significant in vitro positive impact upon cell motility, and an early osteogenic differentiation response in the absence of another stimulus.

Results indicated that the in vivo application of this new ink formulation to regenerate critical size bone defect tends to promote the formation of bone volume fraction without affecting the vascularization of the neo-formed tissue.

The use of LAB with this ink failed to demonstrate a complete bone repair, whether SCAPs were not at its direct proximity.

For the first time, a tricalcium silicate-based printed ink, was characterized in vitro and in vivo, giving valuable information to reach complete bone regeneration.

This LAB process could be the base for many ways to regenerate bones.


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