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Evaluation of keratin biomaterial containing silver nanoparticles as a potential wound dressing in full-thickness skin wound model in diabetic mice

Marek Konop, Joanna Czuwara, Ewa Kłodzińska, Anna K Laskowska, Dorota Sulejczak, Tatsiana Damps, Urszula Zielenkiewicz, Iwona Brzozowska, Antonio Sureda, Tomasz Kowalkowski, Robert A Schwartz, Lidia Rudnicka 

Journal of Tissue Engineering and Regenerative Medicine


Keratin is a cytoskeletal scaffolding protein essential for wound healing and tissue recovery.

The aim of the study was to evaluate the potential role of insoluble fur keratin-derived powder containing silver nanoparticles (FKDP-AgNP) in the allogenic full-thickness surgical skin wound model in diabetic mice. The scanning electron microscopy image evidenced that the keratin surface is covered by a single layer of silver nanoparticles.

Data obtained from dynamic light scattering and micellar electrokinetic chromatography showed three fractions of silver nanoparticles with an average diameter of 130, 22.5, and 5 nm.

Microbiologic results revealed that the designed insoluble FKDP-AgNP dressing to some extent inhibit the growth of Escherichia coli and Staphylococcus aureus.

In vitro assays showed that the FKDP-AgNP dressing did not inhibit fibroblast growth or induce hemolysis. In vivo studies using a diabetic mice model confirmed biocompatible properties of the insoluble keratin dressings. FKDP-AgNP significantly accelerated wound closure and epithelization at Days 5 and 8 (p < .05) when compared with controls.

Wound inflammatory response based on macrophages favors tissue remodeling and healing.

In conclusion, the investigated FKDP-AgNP dressing consisting of an insoluble fraction of keratin, which is biocompatible, significantly accelerated wound healing in a diabetic mouse model.


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